(1) Boyer, R. Biochemistry Laboratory: Modern Theory and Techniques, 2nd ed.; Pearson Education Inc., 2012; pp 289-306. 

(2) Novagen pET system manual 11th edition. w,EMD_BIO-69862 (accessed June 7, 2021). Click on the provided link, click on “user protocol” to access the manual.

(3) Sambrook, J.; Russell, D. W. Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; 2001. 

(4) Luria, S.E. The Recognition of DNA in Bacteria. Scientific American. 1970, 222, 88-92. 1970. 

(5) Smith, H. O.; Wilcox, K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970, 51, 379-91. 

(6) Meselson M.; Yuan R. DNA restriction enzyme from E. coli. Nature. 1968, 217, 1110-1114. 

(7) Nathans, D.; Smith, H. O. Restriction endonucleases in the analysis and restructuring of DNA molecules. Annu. Rev. Biochem. 1975, 44, 273-293. 

(8) Fermentas DoubleDigest engine. (accessed June 7, 2021). 

(9) Thermo Scientific™ GeneRuler 1 kb Plus DNA Ladder

(10) Thorne, H, V. Electrophoretic characterization and fractionation of polyoma virus DNA J. Mol. Biol. 1967, 24, 203-211. 

(11) Keseler, I. M., Mackie, A., Santos-Zavaleta, A., Billington, R., Bonavides-Martínez, C., Caspi, R., … & Karp, P. D. (2017). The EcoCyc database: reflecting new knowledge about Escherichia coli K-12. Nucleic acids research45(D1), D543-D550.

Other references of interest: 

a. Vincze, T.; Posfai, J.; Roberts, R.J. NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res. 2003, 31, 3688-3691. 

b. Barnum, S.R. Biotechnology: Old and New,” and “Basic Principles of Recombinant DNA Technology,” Biotechnology: An Introduction. Thomson, Belmont, California; 2005; pp. 18-24, 58-65, 91-92. 

c. Ninfa, A. J.; Ballou, D.P. (1998) Gel Electrophoresis of DNA, Fundamental Laboratory Approaches for Biochemistry and Biotechnology, Fitzgerald Science Press, Bethesda, Maryland; 1998; pp. 283-286.


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BBS OER Lab Manual Copyright © 2021 by Felicia Vulcu/ Vivian Leong/ Caitlin Mullarkey (Department of Biochemistry and Biomedical Sciences McMaster University) is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, except where otherwise noted.

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