References
(1) Boyer, R. Biochemistry Laboratory: Modern Theory and Techniques, 2nd ed.; Pearson Education Inc., 2012; pp 289-306.
(2) Novagen pET system manual 11th edition. whttps://www.emdmillipore.com/CA/en/product/pET-26b+-DNA-Novagen,EMD_BIO-69862 (accessed June 7, 2021). Click on the provided link, click on “user protocol” to access the manual.
(3) Sambrook, J.; Russell, D. W. Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; 2001.
(4) Luria, S.E. The Recognition of DNA in Bacteria. Scientific American. 1970, 222, 88-92. 1970.
(5) Smith, H. O.; Wilcox, K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970, 51, 379-91.
(6) Meselson M.; Yuan R. DNA restriction enzyme from E. coli. Nature. 1968, 217, 1110-1114.
(7) Nathans, D.; Smith, H. O. Restriction endonucleases in the analysis and restructuring of DNA molecules. Annu. Rev. Biochem. 1975, 44, 273-293.
(8) Fermentas DoubleDigest engine. http://www.thermoscientificbio.com/webtools/doubledigest/ (accessed June 7, 2021).
(9) Thermo Scientific™ GeneRuler 1 kb Plus DNA Ladder
(10) Thorne, H, V. Electrophoretic characterization and fractionation of polyoma virus DNA J. Mol. Biol. 1967, 24, 203-211.
(11) Keseler, I. M., Mackie, A., Santos-Zavaleta, A., Billington, R., Bonavides-Martínez, C., Caspi, R., … & Karp, P. D. (2017). The EcoCyc database: reflecting new knowledge about Escherichia coli K-12. Nucleic acids research, 45(D1), D543-D550.
Other references of interest:
a. Vincze, T.; Posfai, J.; Roberts, R.J. NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res. 2003, 31, 3688-3691.
b. Barnum, S.R. Biotechnology: Old and New,” and “Basic Principles of Recombinant DNA Technology,” Biotechnology: An Introduction. Thomson, Belmont, California; 2005; pp. 18-24, 58-65, 91-92.
c. Ninfa, A. J.; Ballou, D.P. (1998) Gel Electrophoresis of DNA, Fundamental Laboratory Approaches for Biochemistry and Biotechnology, Fitzgerald Science Press, Bethesda, Maryland; 1998; pp. 283-286.
