Writing an Effective Methods Section

Sevda Montakhaby

11 Writing an Effective Methods Section

The Methods section of your thesis paper is much more than just a part of your paper; it’s the foundation that supports your entire research. This section needs to provide a crystal-clear, detailed, and replicable roadmap of your study’s process. It’s a crucial aspect that speaks to the credibility of your work and enables others in the scientific community to replicate your research, a fundamental principle of scientific inquiry.

Many believe, and I concur, that the Methods and Results sections of a paper are somewhat easier to write compared to the more nuanced Introduction and Discussion sections. However, this doesn’t imply that crafting the Methods section is a walk in the park. In reality, it can present its own set of challenges. Due to its technical and descriptive nature, important details can often be missed or misconstrued. The aim of this chapter is to revitalize the Methods section, transforming it from what could be a dry and tedious segment into a compelling and insightful part of your thesis. By doing so, we hope to not only enhance the quality of your thesis but also improve its overall readability and impact.

Bit of a historical fun fact, but the Methods section is traditionally known as the “Materials & Methods” section. The traditional name served to highlight the two crucial aspects of the Methods section.

“Materials” encompasses what was examined in the study, such as human subjects, animals, or tissue samples, as well as various treatments (like drugs or gases) and instruments utilized.
“Methods” dives into the procedural aspects, detailing how subjects or objects were handled to address the experimental question, the methodologies of measurement and calculation, and the techniques used for data analysis.

In writing your Methods section, balance is key. While it’s important to provide enough detail for reproducibility, avoid unnecessary elaboration on well-established procedures. Always aim for clarity, precision, and a logical flow of information. To write a methods section devoid of confusion and ambiguity, a structured approach is often recommended:

Describing the Materials

Begin by detailing the materials used in the study, providing context for the environment in which the research was conducted.

- Assessing the external validity of research involving human subjects hinges on accurately detailing the sample population’s demographic data, including age, gender, and racial composition. This allows for a better understanding of to whom the study results can be applied. Similarly, studies involving animal subjects must specify species, weight, strain, sex, and age. The Methods section should therefore meticulously describe the selection criteria and rationale for participant enrollment, shaping the boundaries of how the results can be generalized. Such detailed reporting ensures a comprehensive understanding of the research scope and enhances the study’s applicability and relevance to wider populations.
- Moreover, when reporting your sample size, you are encouraged to provide a detailed explanation of your power analysis. This approach not only demonstrates the statistical robustness of your study but also justifies the number of participants used. By including this in your Methods section, you provide transparency about how your sample size was sufficient to achieve reliable and valid results, and how it contributes to the study’s overall credibility. This information is key in allowing readers and reviewers to understand the methodological decisions made and ensures the replicability of your research. If the power analysis was conducted post-hoc, this should be clearly stated. Including post-hoc analysis can be insightful, especially in explaining the study’s findings within the context of the actual sample size used. This transparency is vital for readers and reviewers to understand the limitations and strengths of your study’s design and statistical approach.
  - Make sure to specify the tools and statistical packages used for your power analysis. Mention and cite specific software like G-Power or the SuperPower package in R, detailing how they were employed in your study. Additionally, describe the measures used, such as effect size, to give a complete picture of your analysis process. The objective is to provide comprehensive information that enables readers to replicate your power analysis if desired, thereby enhancing the transparency and reproducibility of your research.

Elaborating on Preparation

When detailing your study’s preparation process in the Methods section, it’s essential to provide comprehensive information on how materials were prepared or procured.

If your study involves human participants, you may detail the steps from the moment participants enter the lab to their departure. Key aspects to cover are the process of obtaining consent, the instructions given to participants, and how they were compensated (whether through course credit, monetary payment, or other means). Additionally, it’s important to note whether participants were aware of any manipulations in the study or if they were debriefed afterwards. This level of detail not only enhances the transparency of your research process but also underscores the ethical considerations upheld throughout the study.
For animal studies, a thorough description of the use of sedation, anesthesia, their administration routes, and methods for evaluating their efficacy is necessary. All preparatory steps for animal or tissue use before the research should be described in detail to allow for reproducibility or assessment of their relevance by readers.
- Also, include a statement confirming that your study has received the necessary ethical approval from your Institutional Review Board (IRB). This approval indicates that your study’s protocol aligns with the ethical principles governing research on living subjects. Without this ethical clearance, the integrity and legitimacy of the research are significantly compromised. Not only is conducting the research without such approval ethically questionable, but it also renders the study ineligible for publication in reputable, peer-reviewed scientific journals. The IRB’s approval is a fundamental requirement, ensuring that all research involving human or animal subjects is conducted responsibly and ethically.
In research involving drugs, always use the generic drug name and specify the manufacturer, concentration, dose, and infusion rate. Similarly, if your study uses medical gases, detail their concentration and flow rates.
If you’re employing a standardized preparation procedure or one adapted from another lab or study, and it’s well-documented in a publication easily accessible to your readers, you can maintain brevity in your methods section by citing this reference. This approach directs your readers to a detailed description of the procedure, keeping your section concise. However, it’s crucial to clearly outline any modifications or deviations you made from the original method. This option is recommended primarily when the method you are following is published and you have made only minor alterations. Accurately detailing these changes is important for transparency and helps maintain the integrity of your research.
- Conversely, if your method is particularly unique or unconventional, be as detailed as possible, and consider validating it in a separate publication before submitting the main study. This approach not only establishes credibility but also enhances the reader’s understanding of the novel methodology’s applicability and reliability in your research.

Outlining the Research Protocol

The next step in the Methods section is to expand on the step-by-step procedures, from the initiation of the study to its completion, giving readers a clear roadmap of the experimental journey. Here you should chronologically trace the exact steps of the procedures, starting with baseline conditions and measurements, followed by the alterations of the independent variable and the corresponding measurements of the dependent variable. Additionally, it’s crucial to describe any clinical management aspects during the peri-experimental period not directly controlled by the protocol.

Measurement and Calculation Procedures

Next, specify the variables measured and the methods of measurement. Detailing the measurement instruments is key, including specifics like the manufacturer, model, and any calibration procedures undertaken. Furthermore, explain the rationale behind the choice of measurements, especially when measuring variables indirectly. It’s important to explicitly state when a particular value of a variable represents a specific state or condition. For example, you might specify that “A central venous pressure of ≥ 8 mm Hg was indicative of adequate intravascular volume status.” After describing the measurements, a comprehensive listing of all calculations used in the study should follow. This approach ensures that your research is replicable and that your methodology is transparent and understandable.

Data Analysis

The final step in the Methods section involves detailing how the data will be presented in the Results section. This includes specifying whether data will be represented as means, medians, or other statistical measures. Additionally, it’s important to outline the specific statistical tests that will be used for analyzing inferential data. This section should also define the p-value threshold that will be considered to indicate a statistically significant difference. By clearly describing these aspects, readers can better understand the analytical approach and interpret the findings in the context of the statistical methodologies used.

Practical Tips and Tricks:

Formatting Issues: Check if your department has specific formatting requirements for theses, such as APA, MLA, or Chicago style. This information can usually be found on the department’s website or in the student thesis handbook.
- Make note of any specific guidelines related to font type and size, margin widths, citation style, and structure of the document.
- If departmental guidelines are not specified, arrange a meeting with your thesis supervisor to discuss their expectations regarding formatting. Ask for clarity on details like preferred citation style, headings and subheadings format, and any other specific preferences they might have.
- Pay special attention to the requirements for formatting the methods section. This may include guidelines on presenting figures and tables, describing procedures, and citing sources. Ensure that your current draft of the methods section aligns with these formatting standards.
- It’s significantly more efficient to apply the correct formatting from the beginning rather than revising it later. Start formatting your document as per the guidelines as you draft each section. This approach will save you time and effort and will help maintain consistency throughout your thesis. Utilize available resources such as formatting templates provided by your university or department. Consider using reference management software like EndNote or Zotero to streamline the citation process.
Clarify the Rationale: Begin each segment by explaining why certain methods or materials were chosen. This could range from the choice of a specific chemical compound to your criteria for choosing study samples. Aim for clarity and brevity in your rationale; extensive explanations aren’t necessary. Generally, one to two sentences should be adequate to convey the reasoning behind your procedural choices. This approach keeps your Methods section clear, concise, and focused, preventing it from becoming unnecessarily lengthy. Remember, the goal is to be as informative as possible without overwhelming the reader with excessive detail.
Employ Visual Aids: Diagrams, flowcharts, and tables not only break the text’s monotony but also aid in the reader’s comprehension of complex procedures.
Learn from Existing Literature: This handy tip could significantly streamline your process: Study the methods section in recent publications from your supervisor. These sections are not just academically approved but have also been refined through peer reviews. Use them as a template to shape the structure and tone of your own methods section. However, it’s not about copying; it’s about learning from proven approaches and then adapting them to fit your study’s specific needs. Enhancing readability and transparency should be your goal, so feel free to modify and improve upon these examples. Additionally, exploring papers that employ methods similar to yours, or any well-written methods section you’ve encountered, can offer valuable stylistic insights. Remember, these existing works are not just guides; they’re springboards for your methodological creativity and precision.
Reporting Multiple Experiments: When documenting multiple experiments in your paper, it’s advisable to create distinct Methods sections for each, ensuring clarity and organization. However, if the methodologies across these experiments are largely similar, you can streamline your writing by stating that subsequent experiments followed the same materials, methods, and procedures as outlined initially. In such cases, focus only on detailing any variations or changes specific to each experiment. This approach aids in maintaining the conciseness of your paper, preventing redundancy and avoiding overloading your readers with repetitive information. Remember, the goal is to provide comprehensive yet succinct descriptions, enabling readers to understand the nuances of each experiment without unnecessary repetition.
Subsections: Dividing your Methods section into subsections can significantly enhance its readability and organization. Here are some commonly used subsection titles:
- Study Design: Describing the overall framework of the research.
- Participants: Detailing the demographic and selection criteria for subjects.
- Materials/Equipment: Listing and explaining the tools and materials used.
- Procedure: Outlining the step-by-step process of the experiment or study.
- Data Analysis: Describing statistical methods and analysis techniques.

Common Pitfalls to Avoid:

Overlooking Details: Every aspect of your methodology should be clearly explained. Don’t assume the reader knows anything about your procedures.
Inconsistency: Ensure that the details in your Methods section align with what is presented in the Results and Discussion sections.
Seek Feedback: Seeking feedback is a vital step in refining your Methods section. It’s beneficial to regularly consult with your supervisor and peers, as they can provide valuable insights and help identify areas for improvement. I also recommend taking a step back from your work periodically. When you’re deeply immersed in writing, especially after numerous revisions, you might become accustomed to the content, potentially overlooking areas that need enhancement. By revisiting your Methods section after a break, you approach it with fresh eyes, which can reveal new perspectives. Additionally, consider obtaining feedback from individuals who are less familiar with your study. If they can comprehend your methods easily, it’s a good indication of the section’s clarity and effectiveness.

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Crafting Research Narratives that Transcend Technical Jargon Copyright © 2024 by Sevda Montakhaby Nodeh is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, except where otherwise noted.