5.9: Aggregation of Mitochondrial Nucleoids and mtDNA Damage

Dr. Ju

63 5.9: Aggregation of Mitochondrial Nucleoids and mtDNA Damage

Quant-iT PicoGreen dye can be used to visualise mtDNA damage as a result of increased ROS production. Both treatment groups express brighter fluorescence as a result of potential nuclear envelope damage and therefore reveal increased mtDNA nucleoid aggregation (Zahedi et al., 2019).

Image showing increased fluorescence in both treatment groups, revealing mtDNA damage. Open access image: (Zahedi, A., Phandthong, R., Chaili, A., Leung, S., Omaiye, E., & Talbot, P. (2019). Mitochondrial Stress Response in Neural Stem Cells Exposed to Electronic Cigarettes. IScience, 16, 250–269. doi: 10.1016/j.isci.2019.05.034).

Key Takeaways

Both treatment groups cause increased nuclear envelope damage
This occurs due to increased nucleoid aggregation and mtDNA damage

63 5.9: Aggregation of Mitochondrial Nucleoids and mtDNA Damage

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