7
Annexe 6 : Liste des sites, des acides aminés non canoniques et des plasmides disponibles
| Plasmides d’expression disponibles | Protéine encodée | Résistance aux antibiotiques | Identifiant Addgene |
| pBad CA natif | CA thermostable de type sauvage | ampicilline | 105665 |
| pBad CA TAG20 | CA thermostable TAG20 | ampicilline | 105666 |
| pBad CA TAG93 | CA thermostable TAG93 | ampicilline | 105667 |
| pBad CA TAG97 | CA thermostable TAG97 | ampicilline | 105668 |
| pBad CA TAG126 | CA thermostable TAG126 | ampicilline | 105836 |
| pBad CA TAG186 | CA thermostable TAG186 | ampicilline | 105837 |
| pBad CA TAG233 | CA thermostable TAG233 | ampicilline | 105838 |
| pBad HPII natif | Catalase HPII de type sauvage | ampicilline | 105839 |
| pBad HPII TAG206 | Catalase HPII TAG206 | ampicilline | 105846 |
| pBad HPII TAG392 | Catalase HPII TAG392 | ampicilline | 105848 |
| pBad HPII TAG415 | Catalase HPII TAG415 | ampicilline | 105847 |
| pBad HPII TAG568 | Catalase HPII TAG568 | ampicilline | 105845 |
| pBad HPII TAG283 | Catalase HPII TAG283 | ampicilline | 105843 |
| pBad HPII TAG348 | Catalase HPII TAG348 | ampicilline | 105844 |
| pBad sfGFP | sfGFP | ampicilline | 85482 |
| pBad sfGFP TAG150 | sfGFP TAG150 | ampicilline | 85483 |
CA : anhydrase carbonique II humaine thermostable
HPII : catalase HPII d’E. coli
sfGFP: superfolder green fluorescent protein (protéine fluorescente verte superfolder)
| Enzyme | Site d’insertion de TAG | ncAA pour incorporation |
| CA
Humaine thermostable |
F20 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y |
| F93 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| W97 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| K126 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| E186 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| E233 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| HPII
E. coli |
F206 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y |
| E283 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| K348 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| H392 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| Y415 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y | |
| D568 | 3-NY, pAzF, p-NO2F, pBrF, pAF, pIF, O-CH3Y |
| Plasmide GCE | Machinerie moléculaire | ncAA compatibles pour incorporation | Résistance aux antibiotiques | Identifiant Addgene |
| pDule-para-aminoPhe | pAF | pAF | tétracycline | 85502 |
| pDule-3-nitrotyrosine | NitroY | 3-NY | tétracycline | 85498 |
| pDule-pCNF | pCNF | pAzF, p-NO2F, pBrF, pIF, O-CH3Y | tétracycline | 85494 |
Abréviations des ncAA :
- 3-NY : 3-nitrotyrosine
- pAzF: para-azidophenylalanine
- p-NO2F: para-nitrophénylalanine
- pBrF: para-bromophénylalanine
- pAF: para-aminophénylalanine
- pIF: para-iodophénylalanine
- O-CH3Y : O-méthyltyrosine (synonyme : 4-méthoxy-phénylalanine)
